• Rebuilding Nature. Rebuilding Life.

  • Rebuilding Nature. Rebuilding Life.

  • Rebuilding Nature. Rebuilding Life.

  • Rebuilding Nature. Rebuilding Life.

Functionally Immortalized Alveolar Epithelial Cells

Studying the absorption and toxicity of inhaled drugs or chemicals requires a model reflecting the essential features of the air-blood barrier. For this purpose, InSCREENeX developed a novel alveolar type I cell line using the CI-SCREEN technology (recently published in Nature Communications; Lipps et al., 2018). In a joint collaboration project between the groups from Prof. Dr. Claus-Michael Lehr (Helmholtz Institute for Pharmaceutical Research Saarland (HIPS), Prof. Dr. Dagmar Wirth (Helmholtz Centre for Infection Research (HZI) and InSCREENeX the CI-hAELVi cell line was established and extensively characterized. This work is highlighted in the peer-reviewed paper published by Kuehn et al., 2016.

Specifications of CI-hAELVi

  • Characteristic expression of epithelial cell markers (e.g. Occludin, ZO-1)
  • Type-I-like properties (expression of Caveolin-1, absence of surfactant Prot C)
  • Growth in liquid-liquid as well as air-liquid conditions
  • Tight intercellular junctions and formation of desmosomes
  • Low permeability
  • Transepithelial Electrical Resistance (TEER)-formation
Product Name Cat. No. Information Price
CI-hAELVi
Human Alveolar Epithelial cells
INS-CI-1015 Data sheet Get prices
Related Products
Product Name Cat. No. Size Price
huAEC Medium (ready-to-use) INS-ME-1013-100ml 100 ml € 75.00
huAEC Medium (ready-to-use) INS-ME-1013-500ml 500 ml € 290.00
huAEC Coating solution INS-SU-1018 20 ml € 40.00
huAEC Coating solution INS-SU-1018-100ml 5x20 ml € 160.00
Freezing medium INS-SU-1004 30 ml € 29.20

Related Protocols for Cultivation of CI-hAELVi

Literature

  • Co-culture of human alveolar epithelial (hAELVi) and macrophage (THP-1) cell lines. Kletting S et al., ALTEX. 2017;34(4). doi: 10.14573/altex.1607191. Epub 2017 Nov 23 [link].
  • Human Alveolar Epithelial Cells Expressing Tight Junctions to Model the Air-Blood Barrier. Kuehn A et al., ALTEX. 2016; 33(3):251-60 [link].