Cell Immortalization Service – Primary Mouse Fibroblasts
In addition to our CI-SCREEN Immortalization, we offer the generation of immortalized mouse fibroblasts. This service is routinely applied to primary mouse fibroblasts from embryonal tissue (MEF) or to primary murine fibroblasts from adult mice (for example ear fibroblasts). This approach is based on the stable transduction of the SV40 large T antigen into the desired primary cells. Simply provide us your primary cells and we do the rest. After transduction, cells will be expanded as polyclonal pools and cryopreserved when the newly immortalized cell line displays a robust proliferation pattern (Split ratio 1:5 to 1:10 twice per week).
Time line ♦ 2 months
Price ♦ 799 € per immortalized cell line
We can also offer generation of immortalized MEF cell lines with other expansion genes if e.g. the SV40 large T antigen is interfering with the signaling pathway you are interested in. As an example see the recently published study from Prof. Urs Greber from the University of Zürich.
If desired, we can also isolate the primary cells for you.
Need a quote? Contact us!